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病理诊断免疫组化 淋巴造血组织
1.慢性淋巴细胞白血病或小淋巴细胞淋巴瘤
阳性:CD20,CD43,CD23,CD5
阴性:CD3,CD103,CyclinD1,CD10,FMC7,CD79b
2.套细胞淋巴瘤
阳性:CD20,CD5,CD43,FMC7,CyclinD1,Bcl-2
阴性:CD3,CD10,Bcl-6,CD23
3.套细胞淋巴瘤,母细胞型
阳性:CD20,CD5,CD43,CyclinD1,Bcl-2
阴性:CD3,CD10,CD23
4.套细胞淋巴瘤,多形型
阳性:CD20,CD5,CD43,CyclinD1,Bcl-2
阴性:CD3,CD10,CD23,Bcl-6,MUM-1
5.滤泡淋巴瘤
阳性:CD20,Bcl-2,CD10,Bcl-6
阴性:CD3,CD5,CD23
6.结外边缘区黏膜相关淋巴组织淋巴瘤
阳性:CD20,CD79a,IgM,CD11c
阴性:CD3,CD5,CD10,CD23,CD43
7.结内边缘区淋巴瘤
阳性:CD20,IgM,CD11c
阴性:CD3,CD5,CD10,CD23,CD43
8.淋巴浆细胞性淋巴瘤
阳性:CD20,CD25,CD38,IgM,IgG
阴性:CD3,CD5,CD10,CD103,CD23,IgD
9.毛细胞白血病
阳性:CD20,CD11c,CD103,CD25,CD123,T-bet,Annexin,A1(ANXA1),CD72,FMC7,Cyclin1
阴性:CD3,CD10,CD5
10.Burkitt
阳性:CD20,CD10,Bcl-6
阴性:CD3,CD5,Bcl-2
11.弥漫大B细胞淋巴瘤,非特指
阳性:CD20/CD79a
12.富于T细胞/组织细胞的弥漫大B细胞淋巴瘤
阳性:CD20/CD79a,Bcl-6,少部分病例Bcl-2,EMA
阴性:CD3,CD15,CD30,CD138,EBV
13.原发中枢神经系统的弥漫大B细胞淋巴瘤
阳性:CD20,CD22,CD79a,MUM1(90%),CD10(10%-20%),Bcl-6(60%-80%)
阴性:CD3,GFAP
14.原发皮肤的弥漫大B细胞淋巴瘤,腿型
阳性:CD20,CD79a,Bcl-2,Bcl-6,MUM1,FOX-P1
阴性:CD3,CD10
15.老年性EBV+的弥漫大B细胞淋巴瘤
阳性:CD20,CD79a,MUM1,LMP-1,EBER-ISH
阴性:CD3,CD10,Bcl-6,CD15
16.慢性炎症相关弥漫大B细胞淋巴瘤
阳性:CD20/CD79a,CD30,多数病例呈EBER+
阴性:CD3
17.原发纵隔(胸腺)大B细胞淋巴瘤
阳性:CD20,CD79a,CD30(80%),CD23(70%),EBV。部分表达:Bcl-2,Bcl-6(45%-100%),CD10(8%-32%)
阴性:CD3,CD15,EBV
18.血管内大B细胞淋巴瘤
阳性:CD20,MUM1
阴性:CD3,CD29,CD54
19.ALK阳性大B细胞淋巴瘤
阳性:CD20,ALK,EMA,IgA,CD4,CD57,MUM1
阴性:CD3,CD30,CD45
20.浆母细胞淋巴瘤
阳性:CD79a,CD138/CD38/Vs38c,MUM1,EMA,CD30,IgG,EBER-ISH
阴性:CD3,LMP1
21.起源于HHV8相关多中心Castleman病的大B细胞淋巴瘤
表型CD20+/-,CD79a-,CD138-,CD38-/+
阴性:CD3,LANA1,CD27,EBER-ISH
22.原发渗出性淋巴瘤
阳性:CD45,HLA-DR,CD30,CD38,CD138,VS38,EMA
CD19,CD20,CD79a,CD3,Bcl-6,EBV,LMP1
23.未确定类别的,介于DLBCL与经典霍奇金淋巴瘤之间的B细胞淋巴瘤
阳性:CD45,CD20/CD79a/PAX5,CD30,CD15,OCT-2,BOB.1
阴性:CD3,c(s)Ig,CD10,ALK,Bcl-6表达不定
24.未确定类别的,介于DLBCL与Burkitt淋巴瘤之间的B细胞淋巴瘤
阳性:CD19/CD20/CD79a,CD10,Bcl-6,Bcl-2,MUM-或弱阳性
Ki-67指数50%-100%
25.淋巴瘤样肉芽肿
阳性:CD20,CD30-/+,LMP-/+,EBER-ISH
阴性:CD3,CD15,CD56,Cytotoxic proteins(granzyme B, perforin TIA1)
26.结外NK/T细胞淋巴瘤,鼻型
阳性:CD3ε,CD56,Cytotoxic proteins(granzyme B, perforin TIA1),EBER,部分抗体表达CD30
阴性:CD20,TCRβF1;少数病例表达CD3
Ki-67>60%
27.侵袭新NK细胞白血病
阳性:CD3ε,CD56,CD16,Cytotoxic proteins(granzyme B, perforin TIA1),EBER
阴性:CD20,CD3,TCRβF1,MPO,TdT
28.皮下脂膜炎样T细胞淋巴瘤
阳性:CD3,CD3ε,CD8,Cytotoxic proteins(granzyme B, perforin TIA1),βF1
阴性:CD20,CD4,CD56
29.肠病型T细胞淋巴瘤
阳性:CD8,CD3,CD56+/-,Cytotoxic proteins(granzyme B, perforin TIA1)
阴性:CD20,CD3,βF1,EBER-ISH
30.ALK+间变大细胞淋巴瘤
阳性:CD3-/+,CD30,ALK-1,Cytotoxic proteins(granzyme B, perforin TIA1),EMA(45%-60%)
阴性:CD20
31.ALK-间变大细胞淋巴瘤
阳性:CD3,CD30,Cytotoxic proteins(granzyme B, perforin TIA1),EMA(45%-60%)
阴性:CD20,ALK-1
32.血管免疫母细胞性T细胞淋巴瘤
阳性:CD3,CD4,CD10,CXCL13,Bcl-6;淋巴滤泡外不规则分布的树突状细胞网呈CD21阳性
阴性:CD20
33.母细胞性浆细胞样树突细胞肿瘤,母细胞NK细胞淋巴瘤
阳性:CD4,CD56,CD43,CD68Kp1,CD123;呈部分表达的抗体有TdT,CD68KP
(50%)
阴性:CD20,CD3,CD8,CD30,granzyme B
34.肝脾T细胞淋巴瘤
阳性:CD3,TIA1,CD56+/-,CD8-/+
阴性:CD20,CD4,CD30,TCRβF1,granzymeB,perforin,EBER
35.外周T细胞淋巴瘤,非特指
阳性:CD3,CD4,CD8,CD5-/+,CD7-/+
阴性:CD20,EBER;不见CD21阳性的FDC
36.成人T细胞白血病或淋巴瘤
表型:CD25+,CD3+,CD5+,CD7-,CD4+/CD8-(多数),CD4-/CD8+或CD4+/CD8+,EBER-ISH-
37.淋巴母细胞白血病或淋巴瘤
阳性:TdT,CD99,对于T淋巴母细胞肿瘤:CD3+/-,CD3ε+/-,对于B淋巴母细胞肿瘤:CD20-/+,PAX5+/-
阴性:MPO
38.蕈样霉菌病或Sezary综合征
阳性:CD2,CD3,CD5,CD4,TCRβF1
阴性:CD20,CD56,CD8(绝对多数),CD7(绝对多数),CD30,Cytotoxic proteins(granzyme B, perforin TIA1),EBER-ISH
39.原发皮肤CD30阳性的T细胞性淋巴增生性疾病
阳性:CD30,CD3,TCRβF1
阴性:CD20,CD56,CGP,EMA,ALK-1
40.经典型霍奇金淋巴瘤
阳性:CD30,CD15,EBV,CD20+/-
阴性:CD3,CD45
41.结节性淋巴细胞为主型霍奇金淋巴瘤
阳性:CD20,CD30-/+,CD45
阴性:CD3,CD15,EBV
42.粒细胞肉瘤
阳性:MPO,CD68KP1,CD117,CD45,CD99
阴性:CD68PG-M1,少数可表达TdT,CD34,CD56
43.单核母细胞肉瘤
阳性:CD68KP1,CD68PG-M1,CD45,CD99
阴性:MPO,少数病例可表达TdT,CD56
44.滤泡树突状细胞肉瘤
阳性:CD21,CD23,CD35,CD68KP1+/-,S-100-/+,CD45+/-
阴性:CD1a,Langerin,Ki-67阳性率不定(1%-25%)
45.指状树突细胞肉瘤
阳性:S-100,可部分表达CD45,CD68KP1,lysozyme
阴性:CD21,CD35,CD1a,Langerin,MPO
46.Langerhans细胞组织细胞增生症
阳性:S-100,CD1a,Langerin,CD68KP1,部分病例:CD68KP1,少数病例:CD45,lysozyme
阴性:CD21/CD35
47.肥大细胞增生症
阳性:Traptase,CD117,CD45,CD68KP1
阴性:S-100,CD21/CD23/CD35,MPO,TdT,CD68PG-M1
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