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病理诊断免疫组化 淋巴造血组织

2016-09-05 10:45  阅读(1530)  评论(0)  分类:专业

1.慢性淋巴细胞白血病或小淋巴细胞淋巴瘤

阳性:CD20CD43CD23CD5

阴性:CD3CD103CyclinD1CD10FMC7CD79b

 

2.套细胞淋巴瘤

阳性:CD20CD5CD43FMC7CyclinD1Bcl-2

阴性:CD3CD10Bcl-6CD23

 

3.套细胞淋巴瘤,母细胞型

阳性:CD20CD5CD43CyclinD1Bcl-2

阴性:CD3CD10CD23

 

4.套细胞淋巴瘤,多形型

阳性:CD20CD5CD43CyclinD1Bcl-2

阴性:CD3CD10CD23Bcl-6MUM-1

 

5.滤泡淋巴瘤

阳性:CD20Bcl-2CD10Bcl-6

阴性:CD3CD5CD23

 

6.结外边缘区黏膜相关淋巴组织淋巴瘤

阳性:CD20CD79aIgMCD11c

阴性:CD3CD5CD10CD23CD43

 

7.结内边缘区淋巴瘤

阳性:CD20IgMCD11c

阴性:CD3CD5CD10CD23CD43

 

8.淋巴浆细胞性淋巴瘤

阳性:CD20CD25CD38IgMIgG

阴性:CD3CD5CD10CD103CD23IgD

 

9.毛细胞白血病

阳性:CD20CD11cCD103CD25CD123T-betAnnexinA1(ANXA1)CD72FMC7Cyclin1

阴性:CD3CD10CD5

 

10.Burkitt

阳性:CD20CD10Bcl-6

阴性:CD3CD5Bcl-2

 

11.弥漫大B细胞淋巴瘤,非特指

阳性:CD20/CD79a

 

12.富于T细胞/组织细胞的弥漫大B细胞淋巴瘤

阳性:CD20/CD79aBcl-6,少部分病例Bcl-2EMA

阴性:CD3CD15CD30CD138EBV

 

13.原发中枢神经系统的弥漫大B细胞淋巴瘤

阳性:CD20CD22CD79aMUM1(90%)CD10(10%-20%)Bcl-6(60%-80%)

阴性:CD3GFAP

 

14.原发皮肤的弥漫大B细胞淋巴瘤,腿型

阳性:CD20CD79aBcl-2Bcl-6MUM1FOX-P1

阴性:CD3CD10

 

15.老年性EBV+的弥漫大B细胞淋巴瘤

阳性:CD20CD79aMUM1LMP-1EBER-ISH

阴性:CD3CD10Bcl-6CD15

 

16.慢性炎症相关弥漫大B细胞淋巴瘤

阳性:CD20/CD79aCD30,多数病例呈EBER+

阴性:CD3

 

17.原发纵隔(胸腺)B细胞淋巴瘤

阳性:CD20CD79aCD30(80%)CD23(70%)EBV。部分表达:Bcl-2Bcl-6(45%-100%)CD10(8%-32%)

阴性:CD3CD15EBV

 

18.血管内大B细胞淋巴瘤

阳性:CD20MUM1

阴性:CD3CD29CD54

 

19.ALK阳性大B细胞淋巴瘤

阳性:CD20ALKEMAIgACD4CD57MUM1

阴性:CD3CD30CD45

 

20.浆母细胞淋巴瘤

阳性:CD79aCD138/CD38/Vs38cMUM1EMACD30IgGEBER-ISH

阴性:CD3LMP1

 

21.起源于HHV8相关多中心Castleman病的大B细胞淋巴瘤

表型CD20+/-CD79a-CD138-CD38-/+

阴性:CD3LANA1CD27EBER-ISH

 

22.原发渗出性淋巴瘤

阳性:CD45HLA-DRCD30CD38CD138VS38EMA

CD19CD20CD79aCD3Bcl-6EBVLMP1

 

23.未确定类别的,介于DLBCL与经典霍奇金淋巴瘤之间的B细胞淋巴瘤

阳性:CD45CD20/CD79a/PAX5CD30CD15OCT-2BOB.1

阴性:CD3c(s)IgCD10ALKBcl-6表达不定

 

24.未确定类别的,介于DLBCLBurkitt淋巴瘤之间的B细胞淋巴瘤

阳性:CD19/CD20/CD79aCD10Bcl-6Bcl-2MUM-或弱阳性

Ki-67指数50%-100%

 

25.淋巴瘤样肉芽肿

阳性:CD20CD30-/+LMP-/+EBER-ISH

阴性:CD3CD15CD56Cytotoxic proteins(granzyme B, perforin TIA1)

 

26.结外NK/T细胞淋巴瘤,鼻型

阳性:CD3εCD56Cytotoxic proteins(granzyme B, perforin TIA1)EBER,部分抗体表达CD30

阴性:CD20TCRβF1;少数病例表达CD3

Ki-67>60%

 

27.侵袭新NK细胞白血病

阳性:CD3εCD56CD16Cytotoxic proteins(granzyme B, perforin TIA1)EBER

阴性:CD20CD3TCRβF1MPOTdT

 

28.皮下脂膜炎样T细胞淋巴瘤

阳性:CD3CD3εCD8Cytotoxic proteins(granzyme B, perforin TIA1)βF1

阴性:CD20CD4CD56

 

29.肠病型T细胞淋巴瘤

阳性:CD8CD3CD56+/-Cytotoxic proteins(granzyme B, perforin TIA1)

阴性:CD20CD3βF1EBER-ISH

30.ALK+间变大细胞淋巴瘤

阳性:CD3-/+CD30ALK-1Cytotoxic proteins(granzyme B, perforin TIA1)EMA(45%-60%)

阴性:CD20

 

31.ALK-间变大细胞淋巴瘤

阳性:CD3CD30Cytotoxic proteins(granzyme B, perforin TIA1)EMA(45%-60%)

阴性:CD20ALK-1

 

32.血管免疫母细胞性T细胞淋巴瘤

阳性:CD3CD4CD10CXCL13Bcl-6;淋巴滤泡外不规则分布的树突状细胞网呈CD21阳性

阴性:CD20

 

33.母细胞性浆细胞样树突细胞肿瘤,母细胞NK细胞淋巴瘤

阳性:CD4CD56CD43CD68Kp1CD123;呈部分表达的抗体有TdTCD68KP

(50%)

阴性:CD20CD3CD8CD30granzyme B

 

34.肝脾T细胞淋巴瘤

阳性:CD3TIA1CD56+/-CD8-/+

阴性:CD20CD4CD30TCRβF1granzymeBperforinEBER

 

35.外周T细胞淋巴瘤,非特指

阳性:CD3CD4CD8CD5-/+CD7-/+

阴性:CD20EBER;不见CD21阳性的FDC

 

36.成人T细胞白血病或淋巴瘤

表型:CD25+CD3+CD5+CD7-CD4+/CD8-(多数)CD4-/CD8+CD4+/CD8+EBER-ISH-

 

37.淋巴母细胞白血病或淋巴瘤

阳性:TdTCD99,对于T淋巴母细胞肿瘤:CD3+/-CD3ε+/-,对于B淋巴母细胞肿瘤:CD20-/+PAX5+/-

阴性:MPO

 

38.蕈样霉菌病或Sezary综合征

阳性:CD2CD3CD5CD4TCRβF1

阴性:CD20CD56CD8(绝对多数)CD7(绝对多数)CD30Cytotoxic proteins(granzyme B, perforin TIA1)EBER-ISH

 

39.原发皮肤CD30阳性的T细胞性淋巴增生性疾病

阳性:CD30CD3TCRβF1

阴性:CD20CD56CGPEMAALK-1

 

40.经典型霍奇金淋巴瘤

阳性:CD30CD15EBVCD20+/-

阴性:CD3CD45

 

41.结节性淋巴细胞为主型霍奇金淋巴瘤

阳性:CD20CD30-/+CD45

阴性:CD3CD15EBV

 

42.粒细胞肉瘤

阳性:MPOCD68KP1CD117CD45CD99

阴性:CD68PG-M1,少数可表达TdTCD34CD56

 

43.单核母细胞肉瘤

阳性:CD68KP1CD68PG-M1CD45CD99

阴性:MPO,少数病例可表达TdTCD56

 

44.滤泡树突状细胞肉瘤

阳性:CD21CD23CD35CD68KP1+/-S-100-/+CD45+/-

阴性:CD1aLangerinKi-67阳性率不定(1%-25%)

 

45.指状树突细胞肉瘤

阳性:S-100,可部分表达CD45CD68KP1lysozyme

阴性:CD21CD35CD1aLangerinMPO

 

46.Langerhans细胞组织细胞增生症

阳性:S-100CD1aLangerinCD68KP1,部分病例:CD68KP1,少数病例:CD45lysozyme

阴性:CD21/CD35

 

47.肥大细胞增生症

阳性:TraptaseCD117CD45CD68KP1

阴性:S-100CD21/CD23/CD35MPOTdTCD68PG-M1

 


 

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